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银杏叶提取物促进脑缺血半暗带神经元自噬提高神经保护作用
引用本文:郭涛,吴致远,赵晓明,陈学梅,刘裕源,黄志文,何红云,邓仪昊. 银杏叶提取物促进脑缺血半暗带神经元自噬提高神经保护作用[J]. 中国细胞生物学学报, 2021, 0(3): 552-560
作者姓名:郭涛  吴致远  赵晓明  陈学梅  刘裕源  黄志文  何红云  邓仪昊
作者单位:昆明理工大学医学院解剖学教研室
基金项目:国家自然科学基金(批准号:81960418、81860411);云南省万人计划青年拔尖人才专项(批准号:YNWR-QNBJ-2018-034);云南省应用基础研究计划(批准号:2019FB098、202001AT070049);云南省教育厅科研基金(批准号:2020J0066、2018JS016);省级人培项目(批准号:KKSY201960010)资助的课题。
摘    要:银杏叶提取物(ginkgo biloba extract-761,EGb-761)注射液在中国常作为辅助药物被用于治疗脑卒中,但是,其潜在的细胞和药理机制尚未完全了解.该研究旨在探讨EGb-761是否通过调节缺血性脑卒中半暗带神经元的自噬从而发挥保护作用.采用雄性SD大鼠大脑中动脉闭塞(middle cerebral ...

关 键 词:缺血性脑卒中  EGb-761  自噬  凋亡  神经保护

Ginkgo Biloba Extract Promotes Neuronal Autophagy and Improves Neuroprotection in Penumbra of Cerebral Ischemia
GUO Tao,WU Zhiyuan,ZHAO Xiaoming,CHEN Xuemei,LIU Yuyuan,HUANG Zhiwen,HE Hongyun,DENG Yihao. Ginkgo Biloba Extract Promotes Neuronal Autophagy and Improves Neuroprotection in Penumbra of Cerebral Ischemia[J]. Chinese Journal of Cell Biology, 2021, 0(3): 552-560
Authors:GUO Tao  WU Zhiyuan  ZHAO Xiaoming  CHEN Xuemei  LIU Yuyuan  HUANG Zhiwen  HE Hongyun  DENG Yihao
Affiliation:(Department of Anatomy,Faculty of Medicine,Kunming University of Science and Technology,Stroke Pathological Mechanism Research Laboratory,Kunming 650500,China)
Abstract:EGb-761(ginkgo biloba extract-761)injection has been widely used as an adjuvantive therapy for cerebral stroke in China,but its underlying cellular and pharmacological mechanisms have not been fully understood.This study aims to investigate whether EGb-761 has a therapeutic effect by regulating the autophagy of neurons in the penumbra of ischemic stroke.Using the MCAO(middle cerebral artery occlusion)reperfusion model of male SD rats,the MCAO rats were randomly divided into 5 groups,namely Sham group,MCAO+saline group,MCAO+EGb group,MCAO+EGb+3-MA group and MCAO+3-MA group.Cerebral ischemic rats were injected intraperitoneally with EGb-761 for 7 days,and were injected with autophagy inhibitor 3-MA into the lateral ventricle.The brain tissue of the ischemic penumbra was used to detect the expression of autophagy by WB,qRT-PCR(quantitative real-time PCR)and immunofluorescence.In addition,the evaluation of therapeutic efficacy was based on cerebral infarction volume,neurological deficit,and TUNEL detection of neuronal apoptosis levels.The results showed that compared with MCAO+saline,the EGb-761 drug in the MCAO+EGb group significantly increased the expression of neuronal autophagy.At the same time,EGb-761 treatment significantly reduced the neurological deficit,cerebral infarction area and neuronal apoptosis.In addition,3-MA in the MCAO+EGb+3-MA group counteracted the effect of EGb to enhance neuronal autophagy,and only the use of 3-MA continued to aggravate the nerve damage,compared with the MCAO+EGb group.Therefore,EGB-761 exerts a neuroprotective effect by specifically promoting neuronal autophagy in the penumbra of cerebral ischemia.
Keywords:ischaemic cerebral stroke  EGb-761  autophagy  apoptosis  neuroprotection
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