Hsa_circ_0000745对肝癌细胞增殖、侵袭、迁移的作用及其机制

该文主要研究环状RNA hsa_circ_0000745对肝癌细胞的促肿瘤作用及其相关作用机制.首先,采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)方法检测不同肝癌细胞中环状RNA hsa_circ_0000745的表达;其次,利用siRNA干扰技术敲低HepG2细胞中的...

Effects of hsa_circ_0000745 on Proliferation,Invasion and Migration of Hepatocellular Carcinoma Cells and Its Mechanism

This study was to investigate tumor-promoting effect of circular RNA hsacirc0000745 on hepatocellular carcinoma cells and its related mechanism.Firstly,quantitative Real-time PCR(qRT-PCR)was used to detect the expression of circular RNA hsacirc000745 in hepatocellular carcinoma cells.Secondly,hsa circ000745 in HepG2 cells was knocked down by siRNA interference technology,and then CCK-8 and plate cloning experiments were used to detect the proliferation ability of cells.Transwell test and scratch healing test were used to detect cell invasion and migration ability.Western blot was used to detect the related proteins of proliferation,invasion and migration.The results showed that after interfering with hsacirc0000745,the proliferation and clonogenic ability of hepatocellular carcinoma cells were decreased(P<0.05),and the expression of cyclin D1 was downregulated(P<0.001).The migration and invasion abilities of cells were decreased(P<0.05).The expression of epithelial marker E-Cadherin was up-regulated(P<0.05),and the expressions of interstitial markers N-Cadherin and Vimentin were down-regulated(P<0.05).The results showed that knockdown of circular RNA hsacirc0000745 significantly inhibited the proliferation,migration,invasion and EMT(epithelial-mesenchymal transition)of hepatocellular carcinoma cells,which provided a new potential target and perspective for the diagnosis and treatment of hepatocellular carcinoma.