α-酮戊二酸减轻脂多糖/D-半乳糖胺诱导的小鼠急性肝损伤

该文旨在探讨α-酮戊二酸对脂多糖(lipopolysaccharide,LPS)及D-半乳糖胺(d-galac-tosamine,D-Ga1)诱导的急性肝损伤发生发展的影响及其可能机制.实验分组:正常对照组、AKG单独处理组、LPS/D-Ga1组、LPS/D-Ga1+AKG组.在雄性BALB/c小鼠中,经腹腔注射LPS...

Alpha-Ketoglutarate Attenuates Endotoxin/D-Galactosamine-Induced Acuteliver Injury in Mice

The paper aimed to explore the effect of alpha-ketoglutarate on the occurrence and development of acute liver injury induced by LPS(lipopolysaccharide)and D-Gal(D-galactosamine)and the underlying mechanism.Experimental groups:Control,AKG group,LPS/D-Gal group,LPS/D-Gal+AKG group.In male BALB/c mice,acute liver injury was induced by intraperitoneal injection of LPS/D-Gal.Alpha-ketoglutarate was injected intraperitoneally 0.5 h prior LPS/D-Gal.The mice were sacrificed at 6 h after LPS/D-Gal challenging,and plasma samples were collected for measuring the level of plasma transaminase by colorimetry to evaluate the liver injury.The pathological changes of liver tissue were observed by HE staining.The activities of caspase-3,caspase-8 and caspase-9 were detected by colorimetry.The apoptotic cell was estimated by TUNEL assay,and the level of cleaved caspase-3 was detected by Western blot to imply hepatocyte apoptosis.The results showed that alpha-ketoglutarate-treated LPS/D-Gal-exposed mice had significantly down-regulated incidence of histologic lesions,lower plasma AST(aspartate aminotransferase)and ALT(alanine aminotransferase).Treatment with alpha-ketoglutarate also reduced cleaved caspase-3 and caspase-3,caspase-8,caspase-9 activities and reduced the count of TUNEL-positive hepatocytes.Alpha-ketoglutarate can attenuate the pathological changes of acute liver injury induced by LPS/D-Gal and exert the protective effect of anti-apoptosis.