| 人滑膜间充质干细胞与人脐带间充质干细胞的生物学性状比较 |
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| 引用本文： | 姚映灵,何玲,毕杨,南国新. 人滑膜间充质干细胞与人脐带间充质干细胞的生物学性状比较[J]. 中国细胞生物学学报, 2021, 0(2): 302-310 |
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| 作者姓名： | 姚映灵 何玲 毕杨 南国新 |
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| 作者单位： | 重庆医科大学附属儿童医院骨科二病房;重庆医科大学附属儿童医院儿研所干细胞实验室 |
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| 基金项目： | 国家自然科学基金(批准号:81272172)资助的课题。 |
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| 摘 要： | 该文旨在比较人滑膜间充质干细胞(human synovial mesenchymal stem cells,hSMSCs)与人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)的生物学性状.流式细胞仪鉴定hSMSCs和hUC-MSCs.比较两种间...
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| 关 键 词： | 人滑膜间充质干细胞 人脐带间充质干细胞 多向分化 增殖 迁移 |
Comparison of Biological Characteristics of Human Synovial Mesenchymal Stem Cells and Human Umbilical Cord Mesenchymal Stem Cells |
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| YAO Yingling,HE Ling,BI Yang,NAN Guoxin. Comparison of Biological Characteristics of Human Synovial Mesenchymal Stem Cells and Human Umbilical Cord Mesenchymal Stem Cells[J]. , 2021, 0(2): 302-310 |
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| Authors: | YAO Yingling HE Ling BI Yang NAN Guoxin |
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| Affiliation: | (Department of Orthopaedics Children's Hospital of Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,National Clinical Research Center for Child Health and Disorders(Chongqing),China International Science and Technology Cooperation base of Child development and Critical Disorders,Children's Hospital of Chongqing Medical University,Chongqing 400014,China;Stem Cell Biology and Therapy Laboratory,Chongqing Key Laboratory of Pediatrics,Children's Hospital of Chongqing Medical University,Chongqing 400014,China) |
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| Abstract: | The aim of this study was to investigate the differences in the biological characteristics of hSMSCs(human synovial mesenchymal stem cells)and hUC-MSCs(human umbilical cord mesenchymal stem cells).hUC-MSCs and hSMSCs were identified by flow cytometry.The proliferation,differentiation,migration abilities were comparatively studied between these two MSCs in vitro.The results showed that both hSMSCs and hUC-MSCs showed a long spindle-shaped cell morphology,and flow cytometry analysis showed that both hSMSCs and hUC-MSCs expressed cell surface markers characteristic of MSCs.hUC-MSCs showed high expression of CD73,CD105 and CD147,while hSMSCs showed high expression of CD24.Furthermore,hSMSCs showed more outstanding early osteogenesis and chondrogenesis differentiation abilities.Meanwhile,hUC-MSCs showed stronger proliferation and migration abilities,and the results of cell cycle showed that both hSMSCs and hUC-MSCs had strong proliferation ability.Our findings indicated that hSMSCs and hUC-MSCs presented some similar biological characteristics,and hSMSCs had stronger osteogenic and chondrogenic potentials,which might be used as ideal seed cells for tissue engineering or cells for clinical therapy. |
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| Keywords: | human synovial mesenchymal stem cells human umbilical cord mesenchymal stem cells multi-lineage differentiation proliferation migration |
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