EL转染试剂转染食管鳞癌细胞的条件优化

该文探讨了关于EL转染试剂转染Hsa-miR-6743质粒至食管鳞癌细胞转染效果的影响因素.以食管鳞癌细胞株Eca-109、TE-1和Eca-9706为研究对象,GFP标记的Hsa-miR-6743为报告基因,通过倒置荧光显微镜检测荧光信号优化转染试剂和质粒比值.结果表明,食管鳞癌细胞的种类影响EL转染试剂的转染效果,...

Optimization of Transfection Condition of the EL Transfection Reagent in Esophageal Squamous Cells

This study aimed to explore the influencing factor on the transfection effects of EL transfection reagent,which was used to transfect Hsa-miR-6743 plasmid into esophageal squamous cells.The esophageal squamous cells Eca-109,TE-1 and Eca-9706 were chosen as the research objects.Hsa-miR-6743 marked by GFP(green fluorescent protein)was selected as a reporter gene.The fluorescence signals were detected by inverted fluorescence microscopy to optimize the proportion of transfection reagent and plasmid.The results turned out that the transfection efficiency of EL transfection reagent was influnced by cell types of esophageal squamous cells.The transfection efficiency of EL transfection reagent in Eca-109 cells was better than that in other two cells.The best detection time-point of the optimal transfection efficiency was at 36 h in Eca-109 cells,and cell viability was not affected by EL transfection reagent.The optimal transfection ratio of EL transfection reagent to Hsa-miR-6743 plasmid ranged from 1:4 to 1:2.The transfection efficiency of EL transfection reagent was affected by cell types,the ratio of transfection reagent to plasmid,and transfection time.