| 家蚕耐氟基因RAPD分子标记的筛选及其克隆 |
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| 引用本文： | 徐庆刚,陈克平,姚勤,陆健. 家蚕耐氟基因RAPD分子标记的筛选及其克隆[J]. 生物技术, 2004, 14(3): 10-11 |
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| 作者姓名： | 徐庆刚 陈克平 姚勤 陆健 |
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| 作者单位： | 江苏大学生命科学研究院,江苏,镇江,212013 |
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| 基金项目： | 江苏省农业高新技术项目 (No .BG2 0 0 1 31 7) |
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| 摘 要： | 为获得家蚕耐氟基因的分子标记，以家蚕耐氟品种T6和高敏感品种733新为材料，并构建了其近等基因，采用300个随机引物进行RAPD扩增，获得了与家蚕耐氟性有关的一个分子标记，此标记由引物S250扩增得到，并在F2代分离个体中得到验证，证明了此分子标记的可靠性，进而将此标记克隆进T载体pmd-18中，完成测序，分析发现此序列是未有报道的新序列。
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| 关 键 词： | 家蚕 近等基因 耐氟性 RAPD标记 |
| 文章编号： | 1004-311X(2004)03-0010-02 |
| 修稿时间： | 2003-10-20 |
Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm(Bombyx mori) |
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| Abstract: | In order to find out the molecular marker that linked to the fluoride tolerance gene of the silkworm,we used silkworm strain T6 with tolerant to fluoride and silkworm strain 733xin with highly sensitive to fluoride to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs of these lines.A molecular marker amplified by S250 that linked to the fluoride tolerance gene was obtained.The molecular marker was examined and verified in F2 separated individuals.Subsequently,the molecular marker was cloned into T vector pMD-18 for sequencing.Comparing with sequences available in the GenBank showed that this molecular marker was novel. |
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| Keywords: | Bombyx mori near-isogenic lines fluoride tolerance RAPD marker |
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