A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids |
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| Authors: | D Dean |
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| Institution: | Institute for Enzyme Research, University of Wisconsin, Madison, WI 53706 U.S.A. |
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| Abstract: | A plasmid cloning vector with ampicillin-resistance and streptomycin-sensitivity markers is suitable for the direct selection of strains carrying recombinant plasmids. The selection for plasmid transformants utilizes their ampicillin resistance whereas selection for recombinant plasmids is based on the inactivation of the rpsL gene contained on the plasmid. When streptomycin-resistant Escherichia coli strains are used as recipients in transformation, transformants carrying the parental plasmid are phenotypically sensitive to streptomycin while those carrying hybrid plasmids are resistant to streptomycin. |
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| Keywords: | Recombinant DNA streptomycin sensitivity ampicillin resistance bp base pairs kb kilobase pairs |
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