富含miR-30a的牛初乳组分通过抑制癌细胞自噬促进顺铂化疗的肿瘤消亡

本实验首次发现牛初乳中富含miR-30a.取牛初乳中富含miR-30a的组分处理用顺铂化疗的癌细胞,能显著地抑制癌细胞自噬,促进癌细胞凋亡:单独使用顺铂化疗18 h的癌细胞凋亡率为12.5%,而化疗的同时施加富含miR-30a的牛初乳组分18 h后癌细胞凋亡率为56.5%.用这种富含miR-30a的牛初乳组分饲喂顺铂化疗的荷瘤小鼠,能更有效地抑制肿瘤生长:化疗小鼠饲喂牛初乳组分后,肿瘤直径自6 mm缩小为4.5 mm,而单独用顺铂化疗的肿瘤直径约8 mm.因此富含miR-30a的牛初乳组分能增进化疗药物的疗效.施用牛初乳组分,还可减少化疗药物的用量:单独用顺铂处理细胞18 h需20 mg/L剂量,加牛初乳组分后10 mg/L即可取得明显凋亡效果;单独用顺铂化疗荷瘤小鼠10天每次需500μg,而添加富含miR-30a的牛初乳组分后,顺铂剂量减半即可明显缩小肿瘤.

miR-30a Enriched Colostrum-Fraction Can Inhibit The DDP-induced Autophagy of HeLa Cells and Promote The Tumor Shrink

Here we firstly report that colostrum contains a high level of miR-30a. Using the miR-30a rich fraction extracted from colostrum, we successfully restrained the autophagy activity of HeLa cells induced by DDP, and promoted their apoptosis from 12.5% to 56.5% in 8 h post-treatment of DDP. By feeding the tumor-bearing mice undergoing DDP-chemotherapy with miR-30a- rich fraction from colostrum for 10 days, we reduced tumors size more effective than treating mice with DDP alone. In particular, the diameter of tumors was diminished to 4.5 mm after miR-30a-rich colostrum fraction administration during DDP chemotherapy, while the tumors treated with DDP only grown to 9.0 mm in diameter. In conclusion, the miR-30a-rich fraction of colostrum can sensitize the cancer cells to anti-cancer drugs, thus enhance the effect of chemotherapy drug on tumor treatment.