TheStreptomyces aureofaciens homologue of thewhiB gene is essential for sporulation; Its expression correlates with the developmental stage |
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Authors: | J Kormanec B Ševčíková O Sprušanský O Benada O Kofroňová R Nováková B Řežuchová L Potúčková D Homérová |
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Institution: | (1) Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic;(2) Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic |
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Abstract: | In previous experiments, aStreptomyces aureofaciens gene highly similar to the sporulation-specificwhiB gene ofStreptomyces cœlicolor was identified. By intergrative transformationvia double cross-over, a stable null mutant of thewhiB-homologous gene ofS. aureofaciens was obtained. The disruption blocked differentiation at a stage between the formation of aerial mycelium and the development
of mature spores, producing white aerial hyphae without septation. Expression of thewhiB gene was investigated during differentiation by S1 nuclease mapping, using RNA prepared fromS. aureofaciens in various developmental stages. Two putative promoters were identified upstream of thewhiB coding region. The stronger promoter,whiB-P2, was induced at the beginning of aerial mycelium formation, and the weaker promoter,whiB-P1, was expressed fairly constantly during differentiation. No differences in the expression of thewhiB promoters were detected in anrpoZ-disruptedS. aureofaciens strain. The promoter bearing DNA fragment was inserted into the promoter-probe vector pARC1 to produce an expression pattern
consistent with the results of direct RNA analysis. |
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