In vitro sensitization of human lymphocytes to a myeloma cell-related antigen |
| |
| Authors: | M E Whitson G D Griffin G D Novelli A Solomon |
| |
| Institution: | 1. Department of Biology, University of South Carolina, Columbia, South Carolina 29208, U.S.A.;2. Biology Division. Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, U.S.A.;3. Departments of Medical Biology and Medicine/Memorial Research Center, University of Tennessee Center for the Health Sciences, Knoxville, Tennessee 37920 U.S.A. |
| |
| Abstract: | Peripheral blood lymphocytes from normal human donors were cocultivated with cells from two established human multiple myeloma cell lines, RPMI 8226 and K-737, and with lymphoblastoid cells from a third B cell line, RAMM. After a comparison of three methods of lymphocyte sensitization, a 6-day incubation protocol with equal numbers of normal lymphocytes and mitomycin C-treated tumor cells was selected. Cells from the RPMI 8226 myeloma line stimulated the differentiation of lymphocytes into cytotoxic effector cells as measured by 51Cr release from labeled target cells. The RPMI 8226-sensitized lymphocytes were cytotoxic for myeloma cells (RPMI 8226 and K-737) and for lymphoblastoid cells (RAMM) but not for cells from human lung tumor lines (A549, A427, MB9812), a breast carcinoma line (ALAB), a normal diploid fibroblast line (HSBP), or normal lymphocytes. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
