Affinity chromatography of hyaluronate on glutaraldehyde-fixed SV-3T3 cells |
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| Authors: | R L Goldberg C B Underhill B P Toole |
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| Affiliation: | 1. Section of Pediatric Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109 USA;2. Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA |
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| Abstract: | A simple, rapid colorimetric assay for plasma heparin is presented. The assay employs the metachromasia of azure A when heparin is added. It is useful for 0 to 10 units/ml and does not depend on heparin's anticoagulant activity. Heparin concentrations determined with this assay are not exactly the same as those determined with coagulation assays. This is probably because azure A determines chemical heparin, not anticoagulant active heparin. |
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| Keywords: | To whom correspondence should be addressed. |
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