| 50Hz正弦交变电磁场促进体外培养成骨细胞分化成熟的双“强度窗”效应 |
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| 引用本文： | 周建,陈克明,葛宝丰,程国政,王嘉琪,韦哲. 50Hz正弦交变电磁场促进体外培养成骨细胞分化成熟的双“强度窗”效应[J]. 生物物理学报, 2011, 0(6) |
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| 作者姓名： | 周建 陈克明 葛宝丰 程国政 王嘉琪 韦哲 |
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| 作者单位： | 兰州军区兰州总医院骨科研究所;兰州军区兰州总医院医学工程科; |
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| 基金项目： | 甘肃省科技重大专项资助项目(09ZNKDA025)~~ |
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| 摘 要： | 为研究频率为50 Hz不同强度正弦交变电磁场对体外培养成骨细胞分化及基因表达的影响,体外分离培养大鼠颅骨成骨细胞,传代后随机分为15组,分别用频率50 Hz、强度为0 mT(对照)和0.9～4.8 mT(每组间隔0.3 mT)的正弦交变电磁场处理。发现:正弦交变电磁场处理3～5 d后,成骨细胞呈漩涡样分布;第9 d,1.8和3.6 mT组的碱性磷酸酶活性极显著高于对照组;第0、12、24和96 h,1.5、1.8、3.0和3.6 mT组碱性磷酸酶、骨形态发生蛋白-2和Osterix基因表达水平显著高于对照组;第10 d,1.8和3.6 mT组钙化结节数明显多于对照组。说明50 Hz、0.9～4.8 mT的正弦交变电磁场能促进体外培养成骨细胞分化成熟,并且具有较明显的双强度窗效应,其中1.8和3.6 mT作用最为明显。
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| 关 键 词： | 成骨细胞 电磁场 碱性磷酸酶 骨保护素-2 Osterix |
Double Effect Intensity of 50 Hz Sinusoidal Electromagnetic Fields at Different Intensity on Differentiation and mRNA Expression of Osteoblasts in Vitro |
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| ZHOU Jian,CHEN Keming,GE Baofeng,CHENG Guozheng,WANG Jiaqi,WEI Zhe. Double Effect Intensity of 50 Hz Sinusoidal Electromagnetic Fields at Different Intensity on Differentiation and mRNA Expression of Osteoblasts in Vitro[J]. Acta Biophysica Sinica, 2011, 0(6) |
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| Authors: | ZHOU Jian CHEN Keming GE Baofeng CHENG Guozheng WANG Jiaqi WEI Zhe |
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| Affiliation: | ZHOU Jian1,CHEN Keming1,GE Baofeng1,CHENG Guozheng1,WANG Jiaqi1,WEI Zhe2 1.Institute of Orthopaedics,Lanzhou General Hospital of PLA,Lanzhou 730050,China,2.Institute of Medical Engineering |
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| Abstract: | To investigate the effects of 50 Hz sinusoidal electromagnetic fields(SEMFs) at different intensity on differentiation and gene expression of osteoblasts in vitro.The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 15 groups after one passage.The intensities of the SEMFs were 0 mT(control) and 0.9~4.8 mT(SEMFs groups,interval 0.3 mT) respectively,and the frequency was 50 Hz.The cells were exposed in the SEMFs for 30 min/d.Those without SEMFs exposure were used a... |
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| Keywords: | Osteoblasts Sinusoidal electromagnetic fields Alkaline phosphatase Bone morphogenetic protein-2 Osterix |
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