Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes. |
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Authors: | J A Prez-Gonzalez L H De Graaff J Visser and D Ramn |
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Institution: | J A Pérez-Gonzalez, L H De Graaff, J Visser, and D Ramón |
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Abstract: | Two Aspergillus nidulans genes, xlnA and xlnB, encoding the X22 and X24 xylanases from this fungus, respectively, have been cloned and sequenced. Their cDNAs have been expressed in a laboratory Saccharomyces cerevisiae strain under the control of a constitutive yeast promoter, resulting in the construction of recombinant xylanolytic yeast strains. |
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