| 大腹园蛛拖丝蛋白一级结构初步研究 |
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| 引用本文： | 王迎 谢锦云. 大腹园蛛拖丝蛋白一级结构初步研究[J]. Acta biochimica et biophysica Sinica, 1997, 29(5): 469-474 |
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| 作者姓名： | 王迎 谢锦云 |
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| 作者单位： | 湖南师范大学 |
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| 摘 要： | 采用部分酸水解的方法对大腹园蛛（Ａｒａｎｅｕｓｖｅｎｔｒｏｃｏｓｕｓ）拖丝纤维蛋白进行水解，通过反相高液相色谱分离到一些重复小肽片段，对其序列分析表明，该蜘蛛拖丝蛋白与肖蛸科的棒络新妇蛛（Ｎｅｐｈｉｌａｃｌａｖｉｐｅｓ）丝蛋白的序列比较，有一个完全相同的肽段ＧＹＧＰＧ，其余所测片段测存在差异，显示不同种属的蜘蛛丝在一级结构上的同异，并探讨了结构和功能的关系。
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| 关 键 词： | 蜘蛛 大腹园珠 拖丝蛋白 蛛丝蛋白 一级结构 |
Preliminary Study on the Primary Structure of Dragline Fibroin from the Spider Araneus ventrocosus |
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| WANG Ying,XIE Jin Yun and LIANG Song Ping. Preliminary Study on the Primary Structure of Dragline Fibroin from the Spider Araneus ventrocosus[J]. Acta biochimica et biophysica Sinica, 1997, 29(5): 469-474 |
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| Authors: | WANG Ying XIE Jin Yun LIANG Song Ping |
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| Affiliation: | WANG Ying,XIE Jin Yun and LIANG Song Ping * |
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| Abstract: | The amino acid composition of the dragline fibroin from the spider fibroin of the spider Araneus ventrocosus was analysed and compared with that of the fibroins from different species of spiders. By means of partial acid hydrolysis and high performance liquid chromatography, several peptide fragments of the dragline fibroin were purified.The amino acid sequence analysis showed the sequences of these peptides to be different from that of the fibroin from the spider Nephila clavipes except for one common fragment with the sequence of GYGPG. |
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| Keywords: | Spider fibroin Peptides Primary structure Araneus ventrocosus |
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