Celloidin as a protective film for improving the histochemical localization of succinate dehydrogenase

Synopsis The effect on the localization of succinate dehydrogenase of coating fresh and frozen-dried cryostat sections of unfixed hamster liver with celloidin was examined. It was found that the protective celloidin film not only leads to a more discrete localization of the enzyme, but is also prevents high losses of nitrogenous materials from sections into the incubation medium, a 30% loss in contrast to the 70% with fresh, non-coated sections. Further, after incubation, the morphology of the coated sections is much better than the uncoated ones.