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31P-NMR Study of the Physiological Conditions in Intact Root Cells of Mung Bean Seedlings under Low-Temperature Stress |
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| Authors: | Takeda Yuichi; Ogawa Tomoko; Nakamura Yoshiyuki; Kasamo Kunihiro; Sakata Makoto; Ohta Eiji |
| Institution: | 1Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University Yokohama 223 Japan 2Molecular Function Laboratory, National Food Research Institute Ministry of Agriculture, Forestry and Fisheries Kannondai, Tsukuba, Ibaraki, 305 Japan 3School of Medicine, Kyorin University Mitaka, Tokyo, 181 Japan |
| Abstract: | Intracellular pH and levels of ATP in intact root-tip cellsof mung bean (Vigna mungo L.] Hepper) under low-temperaturestress were investigated in vivo by 31P nuclear magnetic resonance(31P-NMR) spectroscopy. Root-tips of 3 mm in length were excisedfrom seedlings of mung bean that had been chilled at 0°Cafter grown at 30°C. Chilling for longer than 12 h causedchanges in the intracellular pH and decreased levels of ATPin the seedlings. The level of ATP recovered within 30 min butlittle change in pH was observed when samples were rewarmedto 20° C after chilling at 5°C. However, after chillingfor longer than 48 h, neither the intracellular pH nor the levelof ATP was restored. These results suggest that a decline in the activity of tonoplastH+-ATPase, induced by chillings, might be a significant earlyevent in cold-induced injury that leads to cell damage. (Received October 27, 1994; Accepted May 10, 1995) |
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