The effects of heavy metals and metabolic inhibitors on calcium uptake by gills and scales of Fundulus heteroclitus in vitro |
| |
| Institution: | 1. Organic Geochemistry Unit, Key Laboratory of Geoscience Big Data and Deep Resource of Zhejiang Province, School of Earth Sciences, Zhejiang University, Hangzhou, China;2. State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China;3. Southeast Environmental Research Center and Department of Chemistry and Biochemistry, Florida International University, 3000 NE 151st St., North Miami, FL, USA;4. Department of Surface Waters – Research and Management, Swiss Federal Institute of Aquatic Science and Technology (Eawag), Seestrasse 79, Kastanienbaum, Switzerland;5. Department of Earth Sciences, ETH-Zürich, Sonneggstrasse 5, Zürich, Switzerland;6. South Florida Water Management District, West Palm Beach, FL, USA;7. Department of Chemistry and Biochemistry, University of North Carolina Wilmington, Wilmington, NC, USA;1. Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University of Oldenburg, Germany;2. Leibniz Institute for Baltic Sea Research, Warnemünde (IOW), Germany;1. Univ. Bordeaux, Laboratoire d’Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), UMR CNRS 5805, Allée Geoffroy Saint-Hilaire, 33615 Pessac Cedex, France;2. CNRS, Laboratoire d’Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), UMR CNRS 5805, Allée Geoffroy Saint-Hilaire, 33615 Pessac Cedex, France;1. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 511 Kehua Street, Guangzhou 510640, China;2. Key Laboratory of Marine Mineral Resources, Guangzhou Marine Geological Survey, China Geological Survey, Ministry of Natural Resources of the People''s Republic of China, 188 Guanghai Road, Guangzhou 510760, China;3. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China |
| |
| Abstract: | - 1.1. Cadmium (Cd) and zinc (Zn) were inhibitory to calcium uptake by isolated gills of Fundulus heteroclitus in vitro. The metals appeared to act by displacing Ca2+ ions from protein carriers involved in facilitated diffusion.
- 2.2. In saltwater fish, transport of calcium across the serosal membrane of gill chloride cells is partly energy dependent and is likely mediated by Ca2+-ATPase. However, much of the calcium transport through the gill epithelium appears to occur by passive processes.
- 3.3. Cd (10−5M—10−3M) and Zn (10−7M—10−3 M) inhibited calcium uptake by isolated scale patches incubated in a physiological saline.
- 4.4. Cyanide, oubain, and quercetin treatment of scale patches produced results similar to those of the Cd and Zn treatments suggesting that metal-induced inhibition of ATPases may be responsible for reduced calcium transport by scale osteoblasts.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
