Technetium-99m labeled 50H.19 antibody fragments: Interaction of the antibody with platelets |
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| Institution: | 1. IRCCS Ca’ Granda Ospedale Maggiore Policlinico Foundation, Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Milano, Italy;2. Division of Gastroenterology and Hepatology, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano, Italy;3. Division of General Medicine II, ASST Santi Paolo e Carlo, Department of Health Sciences, University of Milan, Milano, Italy;4. Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milano, Italy;5. CRC “A. M. and A. Migliavacca” Center for Liver Disease, Department of Pathophysiology and Transplantation, University of Milano, Milan, Italy;1. Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain;2. Department of Medicine, University of Oviedo, Spain;3. Department of Orthopedics and Trauma Surgery, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain;4. Department of Surgery and Medical Surgical Specialties, University of Oviedo, Spain;5. Centro Regional de Transfusión de Toledo-Guadalajara, Spain;6. Centro Comunitario de Sangre y Tejidos de Asturias, Oviedo, Spain;7. Department of Immunology, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain;8. Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany |
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| Abstract: | The monoclonal antibody 50H.19 recognized three antigens (Mr = 31-, 40-, 45-K) on normal and thromboasthenic platelets, but only one (Mr = 31-K) on Bernard-Soulier platelets. The intact antibody and its F(ab′)2 fragments, had direct platelet-aggregating activity, and induced the platelet release reaction. The intact antibody potentiated platelet aggregation induced by platelet-activating factor or thrombin. Additions of indomethacin did not inhibit aggregation: addition of PGI2, or a calcium channel blocker completely inhibited aggregation. A reduced amount of platelet-aggregating activity was observed with antibody fragments prepared for labeling with 99mTc by pre-exposure to stannous ions, and herein used in biodistribution studies and elsewhere in thrombus imagining studies (J. Nucl. Med. 27: 1315; 1986). Antibody fragments radiolabeled with 99mTc bound to isolated platelets and to clots containing platelets. |
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