Rapid miniaturized chromatography for 111In labeled monoclonal antibodies: Comparison to size exclusion high performance liquid chromatography |
| |
| Institution: | 1. Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Rua Professor Mário Werneck S/N°, Cidade Universitária—Campus da UFMG, Belo Horizonte, MG 31120-970, Brazil;2. Departamento de Análises Clínicas e Toxicológicas—Faculdade de Farmácia, Universidade Federal de Minas Gerais, Cidade Universitária—Campus da UFMG, Belo Horizonte, MG 31270-091, Brazil;1. The University of Tennessee Graduate School of Medicine, 1924 Alcoa Hwy., Knoxville, TN 37920, United States of America;2. University of Tennessee Department of Mechanical, Aerospace, and Biomedical Engineering, United States of America |
| |
| Abstract: | Our laboratory investigated the use of a rapid miniaturized chromatography system, ITLC-SG with 0.9% NaCl, to assess the radiochemical purity of 111In labeled monoclonal antibodies (MoAbs). Radiochemical analysis was performed on numerous 111In labeled antibody preparations with labeling efficiencies ranging from 40 to greater than 95% and the results compared to those obtained with size exclusion high performance liquid chromatography (HPLC). The chromatographic procedure involved challenging radiolabeled antibodies with 0.05 M DTPA to chelate unbound and/or non-specific bound 111In, spotting on miniaturized instant thin layer-silica gel chromatography strips, developing in 0.9% NaCl, and counting appropriate segments for radioactivity. Results of the study demonstrated that the miniaturized chromatography procedure was rapid, taking less than 4 min to complete, and accurate in assessing the amount of unbound or non-specific bound 111In in 111In labeled monoclonal antibodies, when compared to size exclusion HPLC. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
