Alternate fast and slow stepping of a heterodimeric kinesin molecule |
| |
Authors: | Kaseda Kuniyoshi Higuchi Hideo Hirose Keiko |
| |
Institution: | Gene Function Research Center, National Institute of Advanced Industrial Science and Technology and Japan Society for the Promotion of Science, Tsukuba, Ibaraki 305-8562, Japan. |
| |
Abstract: | A conventional kinesin molecule travels continuously along a microtubule in discrete 8-nm steps. This processive movement is generally explained by models in which the two identical heads of a kinesin move in a 'hand-over-hand' manner. Here, we show that a single heterodimeric kinesin molecule (in which one of the two heads is mutated in a nucleotide-binding site) exhibits fast and slow (with the dwell time at least 10 times longer than that of the fast step) 8-nm steps alternately, presumably corresponding to the displacement by the wild-type and mutant heads, respectively. Our results provide the first direct evidence for models in which the roles of the two heads alternate every 8-nm step. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|