Degradation of a Plasticizer, di-n-Butylphthalate by Delftia sp. TBKNP-05 |
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Authors: | Neelakanteshwar K Patil Rajesh Kundapur Yogesh S Shouche T B Karegoudar |
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Institution: | (1) Department of Biochemistry, Gulbarga University, Gulbarga-585 106, Karnataka, India;(2) National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune-411007, India |
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Abstract: | A bacterial strain Delftia sp. TBKNP-05 isolated by para-hydroxybenzoate enrichment technique is capable of degrading di-n-butylphthalate (DBP) as a sole source of carbon and energy. Analysis of intermediates by thin layer chromatography and high
performance liquid chromatography indicated the presence of monobutylphthalate (MBP), phthalate (PA), and protocatechuate
(PCA). The washed cells grown on DBP and PA showed appreciable oxidation of DBP, MBP, PA, and PCA. The enzyme activities in
cell free extracts of Delftia sp. TBKNP-05 exhibited the presence of DBP esterase, MBP esterase, PA-dioxygenase, and protocatechuate 4, 5-dioxygenase.
The protocatechuate is metabolized by a meta-cleavage pathway leading to further mineralization of the compound in this bacterium. |
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