Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati

Leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of Cucumis melo were cultured on a MS medium supplemented with a range and combination of growth regulators. Maximum shoot differentiation from the leaf explants occurred in the combined presence of BAP and 2iP at equimolar concentration of 1 M. Regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. For elongation the shoots were transferred to MS+BAP [1 M]. Such shoots were rooted with 75% frequency on MS+IAA [0.5 M]. The plants have been established in pots.Abbreviations BM Basal Medium - MS Murashige and Skoog - BAP 6-Benzyl amino-purine - 2iP 6- V, V -dimethylallylamino purine - IAA Indole-3-acetic acid