水稻米香基因的遗传分析与精细定位

香味是水稻重要的品质性状之一，由100多种挥发性化合物组成，其中2-乙酰基-1-吡咯啉（2AP）是稻米香气中最主要的成分，且由一对隐性基因（fgr）控制，fgr基因位于水稻第八号染色体上。本研究根据初定位结果，利用籼粳稻基因组序列在RG1／RG28区域内发展高密度的分子标记，结合大分离群体来定位水稻的米香基因。结果显示，fgr基因位于第八条染色体的WJ-7和WJ-8分子标记之间约408kb的区间内。这一结果将对于水稻米香基因的克隆与分离提供了重要依据。

Genetic analysis and fine mapping of candidate genes involved in rice fragrance

Fragrance is one of the most important traits in riee. From more than 100 volatile flavor components, 2-acetyl-l-pyrro- line （2AP） stands out as the key component of aroma in both basmati- and jasmine-style fragrant rice. It has been reported that a single recessive gene （fgr） on chromosome 8 is responsible for the production of 2AP. According to initial mapping results, thefgr region was saturated with high-density markers in the fine-mapping process. After mapping with segregant populations, the result was that the fgr locus was restricted to an interval of 408kb flanked by the left marker WJ-7 and the right marker WJ-8, which will be greatly helpful to fine map and isolate the candidate genes involved in rice fragrance.