In vivo apoptosis of hepatocytes in guinea pigs infected with Leptospira interrogans serovar icterohaemorrhagiae |
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Authors: | Fabrice Merien Johann Truccolo Yannick Rougier Guy Baranton Philippe Perolat |
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Institution: | Leptospira;Laboratory, Institut Pasteur, P.O. Box 61, 98845 Nouméa, New Caledonia Histopathology Laboratory, Institut Pasteur, P.O. Box 61, 98845 Nouméa, New Caledonia;W.H.O. Collaborating Centre on Leptospirosis, Molecular and Medical Bacteriology Unit, Institut Pasteur, 75724 Paris Cedex 15, France |
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Abstract: | To investigate the contribution of the previously demonstrated in vitro apoptosis to the pathogenesis of leptospirosis, guinea pigs were infected with Leptospira interrogans serovar icterohaemorrhagiae strain Verdun and sequentially killed to collect target organs involved in the natural history of the disease (liver, kidneys, lungs, spleen and heart). The combination of histopathological procedures and a specific TUNEL assay showed a significant Leptospira-induced programmed cell death of hepatocytes with a peak at 48 h post inoculation. Hepatocyte nuclei showed morphological changes including fragmented and condensed nuclei. This phenomenon occurred early in the course of the disease at a time where infecting leptospires were present at a low density between the liver parenchyma cells. |
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Keywords: | Leptospira Virulence Apoptosis Hepatocyte In vivo |
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