Isolation and convulsivant effect of the "insulin-like" protein obtained from the exocrine pancreas of Bradypus tridactylus L |
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Authors: | H B Coutinho F J Aguiar E M Freitas A M Souza L M Ferreira R G Pessoa R L Hunter |
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Abstract: | 1. A pancreatic insulin-like protein fraction with low electrophoretic mobility showing high molecular weight is present. 2. Isoelectric focussing studies showed that the high molecular weight protein has a pI about 7.4 when used at a pH range between 3.5 and 8.0. 3. The partially purified aldehyde-fuchsin positive high molecular weight protein fraction gave a positive effect for the convulsivant test in mice. 4. A high molecular weight insulin-like protein in pancreatic juice was found. 5. Insulin was found by radioimmunoassay in the basal and post stimulation pancreatic juice. |
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