Role of MicroRNA Processing in Adipose Tissue in Stress Defense and Longevity |
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| Authors: | Marcelo A Mori Prashant Raghavan Thomas Thomou Jeremie Boucher Stacey Robida-Stubbs Yazmin Macotela Steven J Russell James L Kirkland T Keith Blackwell C Ronald Kahn |
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| Affiliation: | 1. Department of Biochemistry II, University of Göttingen, D-37073 Göttingen, Germany;2. Department of Cardiovascular Physiology, University of Göttingen, D-37073 Göttingen, Germany;3. Department of Neurology, University of Göttingen, D-37073 Göttingen, Germany;4. Institute for Biochemistry and Molecular Biology, Center for Biochemistry and Molecular Cell Science, University of Freiburg, D-79104 Freiburg, Germany;5. Institute for Biology II, Faculty for Biologie, Functional Proteomics, University of Freiburg, D-79104 Freiburg, Germany;6. Center for Biological Systems Analysis, University of Freiburg, D-79104 Freiburg, Germany;7. BIOSS Centre for Biological Signalling Studies, University of Freiburg, D-79104 Freiburg, Germany;8. Medizinisches Proteom-Center, Ruhr-University Bochum, D-44801 Bochum, Germany;9. Department of NanoBiophotonics, Mitochondrial Structure and Dynamics Group, Max-Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany;10. Max-Planck Institute for Biophysical Chemistry, D-37077, Göttingen, Germany |
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| Abstract: | The terminal enzyme of the mitochondrial respiratory chain, cytochrome oxidase, transfers electrons to molecular oxygen, generating water. Within the inner?mitochondrial membrane, cytochrome oxidase assembles into supercomplexes, together with other respiratory chain complexes, forming so-called respirasomes. Little is known about how these higher oligomeric structures are attained. Here we report on Rcf1 and Rcf2 as cytochrome oxidase subunits in S.?cerevisiae. While Rcf2 is specific to yeast, Rcf1 is a conserved subunit with two human orthologs, RCF1a and RCF1b. Rcf1 is required for growth in hypoxia and complex assembly of subunits Cox13 and Rcf2, as well as for the oligomerization of?a subclass of cytochrome oxidase complexes into respirasomes. Our analyses reveal that the cytochrome oxidase of mitochondria displays intrinsic heterogeneity with regard to its subunit composition and that distinct forms of respirasomes can be formed by complex variants. |
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