Factors controlling rates of nonphotochemical transformation of Pisum phytochrome in vitro |
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Authors: | MANABE KATSUSHI; FURUYA MASAKI |
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Institution: | 1Biological Institute, Faculty of Science, Nagoya University Chikusa, Nagoya,464
2Botany Department, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan |
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Abstract: | Nonphotochemical transformations of the far-red absorbing formof phytochrome, such as its decay or dark reversion to Pr, werestudied with solutions obtained from etiolated pea epicotyltissues at various steps of purification. At pH 7.8, the rateof dark Pfr reversion became significantly faster after thecrude extract was purified by gel filtration, but that of wellpurified solutions was quite low. Decay of Pfr was not seenduring any purification step at an alkaline pH, but it occurredin the acidic range of pH even in the presence of sulfhydrylcompounds. The rate of Pfr reversion was also influenced bypH; it increased with an increasing pH. Dark reversion of Pisum Pfr was confirmed to proceed in a short,rapid initial phase followed by a slow phase. (Received September 9, 1970; ) |
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