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Separation of gliadin at pH 3.1 in a polyacrylamide gel suitable for blotting procedures |
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| Authors: | Steffen U. Friis,C. Sch fer-Nielsen |
| Affiliation: | a Department of Biochemistry C, The Panum Institute, University of Copenhagen, 3 Blegdamsvej, DK-2200, Copenhagen N, Denmark b The Protein Laboratory, University of Copenhagen, 34 Sigurdsgade, DK-2200, Copenhagen N, Denmark |
| Abstract: | A polyacrylamide gel electrophoresis system for the separation of gliadin at acidic pH is described. The gel is cast at neutral pH with polymerization in 20 min. Equilibrium of the gel to pH 3.1 takes place during the electrophoresis. The gel is highly uniform with good mechanical properties and therefore suitable for blotting procedures. |
| Keywords: | gliadin gel electrophoresis blotting coeliac disease |
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