Fluorescence characterisation of multiply-loaded anti-HER2 single chain Fv-photosensitizer conjugates suitable for photodynamic therapy. |
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| Authors: | Marina K Kuimova Manpreet Bhatti Mahendra Deonarain Gokhan Yahioglu James A Levitt Ioanna Stamati Klaus Suhling David Phillips |
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| Affiliation: | Chemistry Department, Imperial College London, Exhibition Road, UK. |
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| Abstract: | We report the synthesis, spectroscopic properties and intracellular imaging of recombinant antibody single chain fragment (scFv) conjugates with photosensitizers used for photodynamic therapy of cancer (PDT). Two widely-studied photosensitizers have been selected: preclinical pyropheophorbide-a (PPa) and verteporfin (VP), which has been clinically approved for the treatment of acute macular degeneration (Visudyne). Pyropheophorbide-a and verteporfin have been conjugated to an anti-HER2 scFv containing on average ten photosensitizer molecules per scFv with a small contribution (
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