Macromolecular domains containing nuclear protein p107 and U-snRNP protein p28: Further evidence for an in situ nuclear matrix

Summary Polyclonal antibodies have been produced which react with a nuclear protein having a molecular weight of 107kD and a pl of 8.7–8.8 (designated p107). This protein is shown to be a component of the residual ribonucleoprotein (RNP) network of the nuclear matrix. P107 localized exclusively to the nuclear interior but not within nucleolar or chromatin domains. We have taken advantage of this unique probe to examine whether the RNP network of the isolated nuclear matrix has a physical counterpart in situ. We show that RNA, p107, divalent cations and the 28 kD Sm antigen of U-snRNPs are components of in situ macromolecular assemblies. While the morphology and intranuclear distribution of these assemblies are insensitive to the removal of chromatin, they are markedly altered by degradation of RNA. Digestion in situ of RNA in the presence of EDTA followed by extraction with high ionic strength buffers solubilized the components of these assemblies. Electron microscopic and immunobiochemical data are presented which support the concept that the residual RNP network of the nuclear matrix is an isolate of a pre-existing structure, and that perturbations in this internal network can be created by RNA degradation, depletion of essential metal ions and proteolysis.Abbreviations CRLM polyclonal chicken antibody raised against rat liver nuclear matrix - Sm monoclonal antibody specific for the 28 kd protein antigen of U1, U2, U4, U5 and U6 snRNPs - hnRNP ribonucleoprotein particles containing hnRNA - snRNP ribonucleoprotein particles containing snRNA - PBS phosphate buffered saline - PMSF phenylmethylsulfonyl fluoride - PAGE polyacrylamide gel electrophoresis - EDTA ethylenediaminetetraacetic acid - VRC vanadium ribonucleoside complex - BSA bovine serum albumin - DMSO dimethylsulfoxide - HS high salt buffer - LS low salt buffer