| 温度对Bt水稻及其非靶标害虫褐飞虱生态适应性的影响 |
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| 引用本文： | 杨亚军,吴志红,徐红星,郑许松,田俊策,鲁艳辉,吕仲贤.温度对Bt水稻及其非靶标害虫褐飞虱生态适应性的影响[J].生物安全学报,2016,25(4):279-285. |
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| 作者姓名： | 杨亚军 吴志红 徐红星 郑许松 田俊策 鲁艳辉 吕仲贤 |
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| 作者单位： | 浙江省农业科学院植物保护与微生物研究所浙江省植物有害生物防控重点实验室省部共建国家重点实验室培育基地 |
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| 基金项目： | 国家转基因生物新品种培育重大专项（2014ZX08001-001）；国家水稻产业技术体系（CARS-01-17）；浙江省自然科学基金（LY15C140003） |
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| 摘 要： | 【背景】温度是影响植物生长发育最为重要的环境因子之一。明确温度对Bt水稻及其非靶标害虫的影响有助于今后Bt水稻的推广应用。【方法】设置不同的温度梯度(26、30和34℃)分别处理Bt水稻T2A-1(表达Cry2A蛋白),在处理后不同天数内分别测定Cry蛋白、叶绿素、草酸、可溶性糖和总氮含量,同时评价不同温度处理下Bt水稻上褐飞虱的生态适应性。【结果】Cry蛋白含量显著受温度影响,随着温度升高Cry蛋白含量下降。温度、处理时间及两者相互作用均显著影响Bt水稻T2A-1的叶片叶绿素、叶鞘草酸、可溶性糖和总氮含量。叶绿素含量在26℃处理20 d时高于30、34℃的处理。随着温度的升高,Bt水稻叶鞘草酸含量升高。可溶性糖含量高温(30和34℃)处理后20 d低于26℃处理。Bt水稻叶鞘内可溶性氮含量随着温度的升高而升高。温度对非Bt水稻MH63上叶绿素、草酸、可溶性糖和总氮含量的影响与T2A-1趋势一致。高温(34℃)处理的Bt水稻上的褐飞虱雌雄虫寿命、每雌产卵量及卵孵化率显著低于26℃处理。温度对非Bt水稻MH63上褐飞虱生态适应性参数的影响与T2A-1趋势一致。【结论与意义】温度对Bt水稻具有显著影响,且对Bt水稻上非靶标害虫褐飞虱的生态适应性产生不利影响。本研究有利于今后Bt稻田的害虫综合治理措施的制定。
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| 关 键 词： | 温度 Bt水稻 生理 褐飞虱 生态适应性 |
| 收稿时间： | 2016/3/15 0:00:00 |
| 修稿时间： | 2016/5/23 0:00:00 |
Effects of temperature on Bt rice and ecological fitness of the non-target insect pest Nilaparvata lugens |
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| Ya-jun YANG,Zhi-hong WU,Hong-xing XU,Xu-song ZHENG,Jun-ce TIAN,Yan-hui LU and Zhong-xian L&#;.Effects of temperature on Bt rice and ecological fitness of the non-target insect pest Nilaparvata lugens[J].Journal of Biosafety,2016,25(4):279-285. |
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| Authors: | Ya-jun YANG Zhi-hong WU Hong-xing XU Xu-song ZHENG Jun-ce TIAN Yan-hui LU and Zhong-xian L&#; |
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| Institution: | State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China,State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China and State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China |
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| Abstract: | |
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| Keywords: | temperature Bt rice physiology Nilaparvata lugens ecological fitness |
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