Selection of method for obtaining an active mutanase preparation from Trichoderma harzianum |
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Authors: | Adrian Wiater Janusz Szczodrak |
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Affiliation: | (1) Department of Industrial Microbiology, Maria Curie-Skodowska University, Akademicka 19, 20-033 Lublin, Poland (Fax: + |
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Abstract: | Crude mutanase preparations of Trichoderma harzianum were obtained from the culture supernatant by means of ammonium sulfate salting out, ultrafiltration, freeze-drying, concentration under reduced pressure, and fractional precipitation with organic solvents (methanol, ethanol, propanol, isopropanol, acetone). Ammonium sulfate was the worst precipitant, causing a fall in total mutanase activity by 47%. Other methods of enzyme recovery from the post-culture fluid yielded in most cases very good results in regard to specific and overall activities of the enzymatic preparations. |
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Keywords: | concentration enzymatic preparation enzyme recovery fungal mutanase precipitation |
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