No mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide |
| |
Authors: | Eliana Maria Minicucci Glenda Nicioli da Silva Daniel Araki Ribeiro Daisy Maria Favero Salvadori |
| |
Institution: | 1.Department of Dermatology and Radiotherapy,S?o Paulo State University, UNESP,Botucatu,Brazil;2.Department of Pathology,S?o Paulo State University, UNESP,Botucatu,Brazil;3.Department of Biosciences,Federal University of S?o Paulo, UNIFESP,Santos,Brazil |
| |
Abstract: | The medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. The
present study aimed to investigate mutations in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline
1-oxide (4NQO) using direct DNA-sequencing method. A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide
(4NQO) in drinking water for 4, 12, and 20 weeks at 50 ppm dose. Ten animals were used as negative control. No histopathological
changes in tongue epithelia were observed among controls or in the group treated for 4 weeks with 4NQO. Following 12-week
treatment, hyperplasia and epithelial dysplasia were found in mild and moderate forms. At 20 weeks, the tongue presented moderate
and/or severe oral dysplasia and squamous cell carcinoma, with squamous cell carcinoma in the majority of animals. No mutations
were found in any experimental period evaluated that corresponded to normal oral mucosa, hyperplasia, dysplasia and squamous
cell carcinomas. Taken together, our results suggest that p16CDKN2A mutations in exon 2 are not involved in the multistep
tongue carcinogenesis of Wistar rats induced by 4NQO. |
| |
Keywords: | p16 Rat tongue mucosa Oral squamous cell carcinoma 4-Nitroquinoline 1-oxide Direct DNA-sequencing |
本文献已被 SpringerLink 等数据库收录! |
|