Lactoferrin at basal side of mouse mammary epithelium derives in part from stroma cells |
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Authors: | Chiara Pecorini Serge Delpal Sandrine Truchet Fabienne Le Provost Antonella Baldi Michèle Ollivier-Bousquet |
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Institution: | 1.Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty,Università degli Studi di Milano,Milan,Italy;2.UR909 Nutrition et Régulation lipidique des Fonctions cérébrales,INRA,Jouy-en-Josas,France;3.UR1196 Génomique et Physiologie de la Lactation,INRA,Jouy-en-Josas,France;4.UR339 Génétique Biochimique et Cytogénétique,INRA,Jouy-en-Josas,France |
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Abstract: | Lactoferrin is synthesized by glandular epithelial cells and neutrophils and is also present on both sides of the mammary
epithelium. We have studied the origin of lactoferrin detected in the various compartments of mouse mammary tissue. As revealed
by immunogold electron microscopy, lactoferrin is present in mammary epithelial cells and in the basal region of the epithelium,
associated with connective tissue and stroma cells at all physiological stages studied. A perturbation of protein synthesis
or transport after in vitro treatment with cycloheximide or brefeldin A does not abrogate lactoferrin labelling in the basal
region of the epithelium. The expression of lactoferrin has also been observed in the fat pads of mammary glands from mice
surgically depleted of epithelial cells. The sealing of one teat for 24 h is accompanied by an increase in both the number
of stroma cells and the labelling of myoepithelial cells. Thus, the lactoferrin present in the interstitial space of the mouse
mammary epithelium originates in part from stroma cells. Possible roles of lactoferrin at the basal side of the mammary epithelium
are discussed. |
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