Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov |
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Authors: | Yuan Chun Wang Fang Wang Bao Chao Hou En Tao Wang Wen Feng Chen Xin Hua Sui Wen Xin Chen Yan Li Ya Bo Zhang |
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Institution: | 1. State Key Laboratory for Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico D.F., Mexico;3. Bioyong Technology Co., Ltd., Beijing 100085, China;4. State Forestry Administration Key Laboratory of Biodiversity Conservation in Southwest China, Faculty of Forestry, Southwest Forestry University, Kunming 650224, China;5. Research Institute, Bioengineering Laboratory, Hangzhou Wahaha Group Co., Ltd, Hangzhou 310018, China |
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Abstract: | In a survey of rhizobia associated with the native legumes in Yunnan Province, China, seven and nine strains isolated from the root nodules of Psoralea corylifolia, Sesbania cannabina and Medicago lupulina were respectively classified into the novel genomic species groups I and II in the genus Ensifer (former Sinorhizobium) based on the sequence analyses of the 16S rRNA gene. Analyses of concatenated housekeeping genes (atpD, recA and glnII) further revealed that they were distinct lineages in the genus, and group I was most similar to Ensifer terangae and Ensifer garamanticus (both with 94.2% similarity), while group II was most similar to Ensifer adhaerens (94.0%). These groups could be distinguished from closely related species by DNA–DNA relatedness, MALID-TOF MS, cellular fatty acid profiles and a series of phenotypic characters. Therefore, two novel species were proposed: Ensifer psoraleae sp. nov. (seven strains, type strain CCBAU 65732T = LMG 26835T = HAMBI 3286T) and Ensifer sesbaniae sp. nov. (nine strains, type strain CCBAU 65729T = LMG 26833T = HAMBI 3287T). They had a DNA G + C mol% (Tm) of 58.9 and 60.4, respectively. Both of the type strains formed effective nodules on common bean (Phaseolus vulgaris) and their hosts of origin. In addition, the previously described species Sinorhizobium morelense and Sinorhizobium americanum were renamed as Ensifer morelense comb. nov. and Ensifer americanum comb. nov. according to the accumulated data from different studies. |
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Keywords: | sp" target="_blank">Ensifer psoraleaesp nov sp" target="_blank">Ensifer sesbaniaesp nov comb" target="_blank">Ensifer morelensecomb nov comb" target="_blank">Ensifer americanumcomb nov |
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