Burkholderia pseudomultivorans sp. nov., a novel Burkholderia cepacia complex species from human respiratory samples and the rhizosphere |
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Authors: | Charlotte Peeters James E.A. Zlosnik Theodore Spilker Trevor J. Hird John J. LiPuma Peter Vandamme |
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Affiliation: | 1. Laboratory of Microbiology, Ghent University, Ghent, Belgium;2. Centre for Understanding and Preventing Infection in Children, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada;3. Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA |
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Abstract: | Eleven Burkholderia cepacia-like isolates of human clinical and environmental origin were examined by a polyphasic approach including recA and 16S rRNA sequence analysis, multilocus sequence analysis (MLSA), DNA base content determination, fatty acid methyl ester analysis, and biochemical characterization. The results of this study demonstrate that these isolates represent a novel species within the B. cepacia complex (Bcc) for which we propose the name Burkholderia pseudomultivorans. The type strain is strain LMG 26883T (=CCUG 62895T). B. pseudomultivorans can be differentiated from other Bcc species by recA gene sequence analysis, MLSA, and several biochemical tests including growth at 42 °C, acidification of sucrose and adonitol, lysine decarboxylase and β-galactosidase activity, and esculin hydrolysis. |
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Keywords: | Bcc, Burkholderia cepacia complex MLSA, multilocus sequence analysis CF, cystic fibrosis |
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