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Burkholderia pseudomultivorans sp. nov., a novel Burkholderia cepacia complex species from human respiratory samples and the rhizosphere
Authors:Charlotte Peeters  James E.A. Zlosnik  Theodore Spilker  Trevor J. Hird  John J. LiPuma  Peter Vandamme
Affiliation:1. Laboratory of Microbiology, Ghent University, Ghent, Belgium;2. Centre for Understanding and Preventing Infection in Children, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada;3. Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA
Abstract:Eleven Burkholderia cepacia-like isolates of human clinical and environmental origin were examined by a polyphasic approach including recA and 16S rRNA sequence analysis, multilocus sequence analysis (MLSA), DNA base content determination, fatty acid methyl ester analysis, and biochemical characterization. The results of this study demonstrate that these isolates represent a novel species within the B. cepacia complex (Bcc) for which we propose the name Burkholderia pseudomultivorans. The type strain is strain LMG 26883T (=CCUG 62895T). B. pseudomultivorans can be differentiated from other Bcc species by recA gene sequence analysis, MLSA, and several biochemical tests including growth at 42 °C, acidification of sucrose and adonitol, lysine decarboxylase and β-galactosidase activity, and esculin hydrolysis.
Keywords:Bcc, Burkholderia cepacia complex   MLSA, multilocus sequence analysis   CF, cystic fibrosis
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