Colony-forming and colony-stimulating cells in normal human peripheral blood |
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| Authors: | Marta Granstr m,G. Gahrton |
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| Affiliation: | 1. Department of Virology, Statens Bakteriologiska Laboratorium, Stockholm, Sweden;2. Institute for Medical Cell Research and Genetics, Medical Nobel Institute, Karolinska Institutet Stockholm, Sweden;3. The Department of Medicine, Karolinska Sjukhuset, Stockholm, Sweden |
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| Abstract: | Peripheral white blood cells (WBC) from normal persons form colonies of granulocytic cells in vitro in soft agar. Stimulus was provided by a feeder layer of peripheral WBC. By centrifugation through an Isopaque-Ficoll gradient, the cells were separated into a mononuclear and a granulocytic fraction with a purity of 96–98% in each fraction. Both the colony-forming cells and the cells inducing colony formation were found in the mononuclear cell fraction. Further fractionation of these mononuclear cells on adherence glass bead columns showed that the colony-forming and colony-inducing cells do not belong to the small lymphocyte population, but were found in the glass adherent fraction containing large monocyte-like and atypical mononuclear cells. |
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