Differential expression of P2Y receptors in the rat cochlea during development |
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Authors: | Lin-Chien Huang Peter R Thorne Srdjan M Vlajkovic Gary D Housley |
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Institution: | (1) Department of Physiology & Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand;(2) Discipline of Audiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand;(3) Department of Physiology & Translational Neuroscience Facility, School of Medical Sciences, University of New South Wales, Sydney, Australia;(4) Translational Neuroscience Facility, School of Medical Sciences, UNSW Kensington Campus, University of New South Wales, Sydney, NSW, 2052, Australia; |
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Abstract: | Purinergic signaling has broad physiological significance to the hearing organ, involving signal transduction via ionotropic
P2X receptors and metabotropic G-protein-coupled P2Y and P1 (adenosine), alongside conversion of nucleotides and nucleosides
by ecto-nucleotidases and ecto-nucleoside diphosphokinase. In addition, ATP release is modulated by acoustic overstimulation
or stress and involves feedback regulation. Many of these principal elements of the purinergic signaling complex have been
well characterized in the cochlea, while the characterization of P2Y receptor expression is emerging. The present study used
immunohistochemistry to evaluate the expression of five P2Y receptors, P2Y1, P2Y2, P2Y4, P2Y6, and P2Y12, during development of the rat cochlea. Commencing in the late embryonic period, the P2Y receptors studied were found in
the cells lining the cochlear partition, associated with establishment of the electrochemical environment which provides the
driving force for sound transduction. In addition, early postnatal P2Y2 and P2Y4 protein expression in the greater epithelial ridge, part of the developing hearing organ, supports the view that initiation
and regulation of spontaneous activity in the hair cells prior to hearing onset is mediated by purinergic signaling. Sub-cellular
compartmentalization of P2Y receptor expression in sensory hair cells, and diversity of receptor expression in the spiral
ganglion neurons and their satellite cells, indicates roles for P2Y receptor-mediated Ca2+-signaling in sound transduction and auditory neuron excitability. Overall, the dynamics of P2Y receptor expression during
development of the cochlea complement the other elements of the purinergic signaling complex and reinforce the significance
of extracellular nucleotide and nucleoside signaling to hearing. |
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Keywords: | Purinergic signaling Cochlear ontogeny ATP Spiral ganglion Hair cells |
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