Population-based resequencing analysis of improved wheat germplasm at wheat leaf rust resistance locus Lr21 |
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Authors: | Yong-Bi Fu Gregory W Peterson Brent D McCallum Li Huang |
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Institution: | 1. Department of Plant Breeding, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Giessen, 35392, Germany 2. Department of Crop Sciences, Georg-August-University G?ttingen, Von-Siebold-Str. 8, G?ttingen, 37075, Germany
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Abstract: | Little is known about the genetic control of heterosis in the complex polyploid crop species oilseed rape (Brassica napus L.). In this study, two large doubled-haploid (DH) mapping populations and two corresponding sets of backcrossed test hybrids
(THs) were analysed in controlled greenhouse experiments and extensive field trials for seedling biomass and yield performance
traits, respectively. Genetic maps from the two populations, aligned with the help of common simple sequence repeat markers,
were used to localise and compare quantitative trait loci (QTL) related to the expression of heterosis for seedling developmental
traits, plant height at flowering, thousand seed mass, seeds per silique, siliques per unit area and seed yield. QTL were
mapped using data from the respective DH populations, their corresponding TH populations and from mid-parent heterosis (MPH)
data, allowing additive and dominance effects along with digenic epistatic interactions to be estimated. A number of genome
regions containing numerous heterosis-related QTL involved in different traits and at different developmental stages were
identified at corresponding map positions in the two populations. The co-localisation of per se QTL from the DH population
datasets with heterosis-related QTL from the MPH data could indicate regulatory loci that may also contribute to fixed heterosis
in the highly duplicated B. napus genome. Given the key role of epistatic interactions in the expression of heterosis in oilseed rape, these QTL hotspots might
harbour genes involved in regulation of heterosis (including fixed heterosis) for different traits throughout the plant life
cycle, including a significant overall influence on heterosis for seed yield. |
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