Basic fibroblast growth factor in rat salivary glands |
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Authors: | Osamu Amano Yoshino Yoshitake Katsuzo Nishikawa Shoichi Iseki |
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Institution: | (1) Department of Anatomy, School of Medicine, Kanazawa University, 13-1 Takara-machi, 920 Kanazawa, Ishikawa, Japan;(2) Department of Biochemistry, Kanazawa Medical University, 920-02 Uchinada, Ishikawa, Japan |
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Abstract: | We studied the occurrence and localization of basic fibroblast growth factor (bFGF) in rat salivary glands using a specific monoclonal antibody. It was shown that the extract of rat salivary glands has a pronounced stimulatory activity on the growth of bovine capillary endothelial cells, which is blocked by the addition of an antibody against bFGF. The concentration of bFGF in the submandibular/sublingual gland, as determined by radioimmunoassay, was 80% that in the brain. Immunocytochemistry revealed bFGF-immunoreactivity localized primarily in the epithelial cells lining the striated ducts and excretory ducts of the parotid, sublingual and submandibular glands. In addition, intense bFGF-immunoreactivity was observed in the granular convoluted tubule of the submandibular gland, localized predominantly in the agranular pillar cells, which lay in small numbers among the majority of weakly immunostained cells containing many apical secretory granules. At the electron-microscopic level, the immunoreactive material was distributed diffusely in the cytoplasmic matrix and nuclei of all immunoreactive cells, whereas it was absent from all cytoplasmic organelles including the secretory granules. These results indicate that bFGF is localized in different cellular and subcellular compartments from those of other growth factors in the duct system of rat salivary glands. |
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Keywords: | Basic fibroblast growth factor Salivary glands Cell growth assay Immunohistochemistry Radioimmunoassay Rat (Wistar) |
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