| 一种能表现水稻基因组DNA分化特征的重复DNA顺序 |
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| 引用本文： | 刘钿连 梁能. 一种能表现水稻基因组DNA分化特征的重复DNA顺序[J]. Acta Botanica Sinica, 1994, 36(6): 452-458 |
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| 作者姓名： | 刘钿连 梁能 |
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| 作者单位： | 中山大学生物工程研究中心!广州510275(刘钿连，王春新，刘良式)，广东省农业科学院水稻研究所!广州510640(梁能) |
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| 基金项目： | 国家863 计划，美国洛氏基金 |
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| 摘 要： | 用ＤＮＡ 复性动力学方法克隆到一个水稻中度重复顺序。Ｓｏｕｔｈｅｒｎ 杂交、限制性内切酶分析及序列分析资料表明，该重复顺序在水稻基因组中具有串联重复和散布状态两种存在方式。以该ＤＮＡ 片段作探针，用Ｓｏｕｔｈｅｒｎ 杂交方法分析了多种野生稻种和栽培稻品种的基因组分化特征。某些限制性内切酶消化过的水稻ＤＮＡ，其图谱呈现出多达４０ 条以上的杂交带，包括强杂交带和弱杂交带两种类型。重复实验结果证明，强杂交带表现为ＢＢＣＣ染色体组型特异而弱带则在栽培稻各品种间显示出丰富的多态性，表明该重复顺序片段在水稻理论研究和育种实践中可能具有重要意义
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| 关 键 词： | 水稻 重复DNA顺序 分子探针 |
A REPETITIVE DNA SEQUENCE SHOWING THE CHARACTE RISTIC OF GENOMIC DNA DIFFERENTIATION IN ORYZA |
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| Liu Dian lian,Wang Chun xin and Liu Liang shi. A REPETITIVE DNA SEQUENCE SHOWING THE CHARACTE RISTIC OF GENOMIC DNA DIFFERENTIATION IN ORYZA[J]. , 1994, 36(6): 452-458 |
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| Authors: | Liu Dian lian Wang Chun xin Liu Liang shi |
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| Abstract: | A moderately repetitive DNA sequence from rice had been cloned by the method of DNA renaturation kinetics.Restriction site analysis, Southern hybridization and DNA sequence analysis indicated that the sequence was mainly in tandem repeat in rice genome.A lot of wild and cultivated rice species were analyzed by Southern blot.With some restriction endonucleases digestion the hybridization pattern of rice DNA showed more than 40 bands with different intensity mainly consisting of the strong and weak hybridization bands.It was found that the strong hybridization bands were specific to the BBCC genome and the weak bands showed abundent polymorphism in cultivated rice species.These results suggested that the repetitive DNA sequence might be useful for rice breeding. |
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| Keywords: | Rice Repetitive DNA sequence Molecular probe |
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