百合离体生殖细胞骨架的扫描电镜观察

从百合的花粉管分离出来的生殖细胞，经表面活化剂Ｔｒｉｔｏｎ Ｘ－１００ 及核糖核酸酶、硫酸铵离析处理。离析后的细胞经临界点干燥，扫描电镜观察显示：在离体生殖细胞的胞质内有一个非常复杂的支架网络系统。这一网络系统有内外两层：外层（靠近细胞膜）网络结构紧密，纤维束粗长；内层（靠近核）网络结构疏松，纤维束短细。一些间接证据显示，这一支架网络系统可能为微管骨架

SCANNING ELECTRON MICROSCOPIC STUDY OF CYTOSKE LETON IN ISOLATED GENERATIVE CELLS OF LILY

Isolated generative cells of lily were extracted with Triton X 100, ammonium sulphate and RNase.The exposed contents were then viewed in the scanning electron microscope after critical point drying.The treatments revealed that in the cytoplasm of the generative cell there was a reticulate network of cytoskeleton.This reticulate network of cytoskeletal scaffold had two layers:(1)an outer layer(near the membrane) consisting of long and thick fibres that were tightly knitted together, and (2) an inner layer (near the nucleus) consisting of thin and short fibres that were loosely knitted together. Indirect evidences using immunofluorescence techniques for labelling microtubules and TRITC phalloidin staining of actin microfilaments indicated that the cytoskeleton seen in the scanning electron microscope appeared likley to be a microtubule cytoskeleton rather than a microfilament cytoskeleton.