高产人参寡糖素培养细胞克隆系的筛选

人参（ＰａｎａｘｇｉｎｓｅｎｇＣ．Ａ．Ｍｅｙ．）培养细胞经细胞平板克隆，获得近３００个克隆系。克隆系在细胞生长速率和寡糖素含量及产率上均存在显著差异，且寡糖素产率和细胞生长之间有明显的相关性。经１１代连续继代培养观察及过氧化物酶同工酶谱特征分析，筛选到一株寡糖素产率高且稳定的克隆系ＰＧ－１８０，其平均生长速率是０．４９５ｇ于重／Ｌ·天，为原始株系的１．３９倍，平均寡糖素含量为１４．６９％干重，是亲本的１．６５倍，平均寡糖素产率是２．１８３ｇ／Ｌ，为原始株系的２．３２倍。比较克隆系ＰＧ－１８０和原始株系细胞悬浮培养时间进程发现，由人参培养细胞生产寡糖素的最佳细胞收获期为３周左右。

SCREENING OF CLONE LINES WITH HIGH YIELD OFOLIGOSACCHARINS FROM CULTURE CELLSOF PANAX GINSENG

Cell plating clone technique was employed to screen clone lines with high yield ofoligosaccharins from culture cells of Panax ginseng C. A. Mey. Near 300 clone lines wereobtained. The results from some clone lines analysed implied that these clone lines were significantly different in cell growth rate, oligosaccharins content and yield. Furthermore, therewas a distinct correlation between oligosaccharins productivity and cell growth. A more stable high-yield oligosaccharin clone line PG-180 had been selected according to the character-characteristics of growth rate, oligosaccharin yield and peroxidases lsozyme patterns during successivesubculturing of 11 generations of clone lines. The mean growth rate of clone line PG-180 was0. 495 g dry wt/L. d, and was 1. 39 folds higher than to the original strain. Its mean content and yield of oligosaccharins were 14. 69 % dry wt and 2. 183 g/l-, which were 65 % and132% respectively higher than those of 'the original strain. In comparing the time course ofcell suspension culture between clone line PG-180 and the original strain. the optimal periodfor high oligosaccharin production from P. ginseng culture cells was approximately threeweeks.