| 两种杆状病毒的限制性消化和p10基因的定位 |
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| 引用本文： | 黄永秀,齐义鹏,李凌云,金天全.两种杆状病毒的限制性消化和p10基因的定位[J].微生物学报,1994,34(3):191-197. |
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| 作者姓名： | 黄永秀 齐义鹏 李凌云 金天全 |
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| 作者单位： | 武汉大学病毒学研究所；武汉大学病毒学研究所 武汉 |
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| 基金项目： | 国家自然科学基金，国家教委博士点基金，国家“八五”攻关课题资助项目 |
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| 摘 要： | 以5和6种限制性内切酶分别消化昆虫杆状病毒SeNPV和LsNPV DNA,求出它们的基因组平均长133kb和164kb.为了定位这两种杆状病毒的p10基因,构建了含有AcNPVp10基因序列的两个探针载体pAcHP106和pAcEP102.从探针载体回收0.18kb和0.42kb的AcNPV p10基因序列,制备探针,与SeNPV和LsNPV的酶切片段杂交,得到清晰的杂交图谱,准确的定位了这两种病毒的p10基因.
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| 关 键 词： | 杆状病毒 P10 基因 基因定位 Baculovirus p10 gene Gene location Probe vector |
RESTRICTION DIGESTION OF TWO KIND OF BACULOV-IRUSES AND THE LOCATION OF THEIR p10 GENES |
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| Huang Yongxiu Qi Yipeng Li Lingyun Jin Tianquan.RESTRICTION DIGESTION OF TWO KIND OF BACULOV-IRUSES AND THE LOCATION OF THEIR p10 GENES[J].Acta Microbiologica Sinica,1994,34(3):191-197. |
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| Authors: | Huang Yongxiu Qi Yipeng Li Lingyun Jin Tianquan |
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| Abstract: | The length of SeNPV and LsNPV genomes DNA were calculated to be 133kb and 164kb respectively, according to digestion with 5-8 restriction endonuleases. In order to map p10 gene in Baculoviruses DNA by pure probe, two probe vectors, pAcHP106 and pAcEP102,were constructed with 3'-0. 18kb and 3'-0. 18kb 5'-0. 24kb (coding sequence and promoter) from AcNPV p10 gene, The p10 genes of both baculoviruses,SeNPV and LsNPV,are located with 0. 18kb and 0. 42kb probes produced by random primer labeling. |
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| Keywords: | Baculovirus p10 gene Gene location Probe vector |
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