圈卷产色链霉菌分化及其特性的研究

链霉菌分化的分子生物学研究是一个饶有兴趣并富有挑战性的世界前沿研究课题.在原核生物的分化研究中,主要以枯草杆菌(Bacillus subtilis)作为模式系统,但枯草杆菌的生命周期尤其是分化过程远比链霉菌简单,不象链霉菌有基质菌丝、气生菌丝、菌丝螺旋和孢子分隔那样的发育分化过程[1].在真核生物中也有分化研究的报道,如构巢曲霉、酵母等.由于真核生物的基因结构比原核生物复杂得多,弄清分化中基因调控的关系就更加困难.因此,选用链霉菌作分化研究的材料有其独一无二的优越性.国际上有关链霉菌的分子生物学研究,近年来主要以链霉菌的抗生素生物合成基因和链霉菌的分化基因两个大的方面作为研究的热点和主攻方向,并展开了一些开拓性的研究.

STUDIES ON THE DIFFERENTIATION AND PROPERTIES OF STREPTOMYCES ANSOCHROMOGENES

S. ansochromogenes is a Nikkomycin producer which isolated from soil in northeast of China. It has typical differentiation properties on glucose-asparagine medium. After pre-germination spores were treated with NTG, seven white mutants (whi)were obtained, which could not produce mature grey spores, and also eight bald mutants (bld) could not produce aerial mycelium. After several generations, all mutants are stable, bld mutants and some of the whi mutants could not produce nikkomycin. It shows that the blocked genes function not only in morphological differentiation but also in antibiotic biosynthesis. 5. ansochromo genes is a suitable material for the study on molecular biology of differentiation, especially, the