Isolation of cDNA clones encoding the human Sm B/B′auto-immune antigen and specifically reacting with human anti-Sm auto-immune sera |
| |
| Authors: | N G Sharpe D G Williams D N Howarth B Coles D S Latchman |
| |
| Institution: | Medical Molecular Biology Unit, University College, London, England. |
| |
| Abstract: | A cDNA clone for the human SmB and B′ auto-immune antigens has been isolated by antibody screening of a cDNA expression library. The cDNA clone hybridises with two distinct mRNAs, one of which is expressed in a tissue-specific manner. A fusion protein expressed from the cDNA clone was recognised by a number of sera from systemic lupus erythematosus (SLE) patients containing anti-Sm antibodies but not by sera reactive with other auto-immune antigens. The potential use of this clone in a diagnostic assay for SLE and in elucidating the processes regulating the expression of SmB and B′ is discussed. |
| |
| Keywords: | Protein SmB Protein B′ Autoimmune antigen Systemic lupus erythematosus RNA splicing alternative |
| 本文献已被 ScienceDirect 等数据库收录！ |
