Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate |
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| Authors: | LG?Sokolovskaya LV?Sigolaeva AV?Eremenko IV?Gachok GF?Makhaeva NN?Strakhova VV?Malygin RJ?Richardson Email author" target="_blank">IN?KurochkinEmail author |
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| Institution: | (1) Faculty of Chemistry, Moscow State University, 119899 Moscow, Russia;(2) Institute of Physiologically Active Compounds, Russian Academy of Sciences, 142432 Moscow Region, Chernogolovka, Russia;(3) Toxicology Program, University of Michigan, 48109 Ann Arbor, Michigan, USA |
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| Abstract: | A graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected at 25 nM (S/N=2) using an Ag/AgCl reference electrode. The biosensor was used to measure the activity of a toxicologically significant enzyme, neuropathy target esterase (NTE), which yields phenol by hydrolysis of the substrate, phenyl valerate. Using the new biosensor, blood and brain NTE inhibition by organophosphorus (OP) compounds with different neuropathic potencies were well correlated (r=0.990, n=7), supporting the use of blood NTE as a biochemical marker of exposure to neuropathic OP compounds. |
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| Keywords: | biosensor mediator 1-methoxyphenazine methosulfate neuropathy target esterase tyrosinase |
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