Coccus-shaped Bacillus subtilis cells are inhibited at stage 0 of sporulation |
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Authors: | H Y Cheung L Vitkovi? |
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Institution: | Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892. |
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Abstract: | RodA and rodB mutations cause rod-shaped Bacillus subtilis cells to become coccus-shaped when the growth temperature is increased from 30 to 45 degrees C. At 30 degrees C four rod strains sporulated as well as the genetically closely related rod+ strains. In contrast, at 45 degrees C the sporulation frequencies of rod strains decreased approximately 10(2)- to 10(4)-fold, while those of rod+ strains remained either unchanged or decreased only slightly. Temperature shift experiments and ultrastructural data indicated that coccus-shaped cells were unable to form prespore septa and were, therefore, inhibited at stage 0 of sporulation. |
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