Proteomic analysis of human papillomavirus‐related oral squamous cell carcinoma: Identification of thioredoxin and epidermal‐fatty acid binding protein as upregulated protein markers in microdissected tumor tissue |
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| Authors: | Christian Melle Günther Ernst Robert Winkler Bettina Schimmel Jens Peter Klussmann Claus Wittekindt Orlando Guntinas‐Lichius Ferdinand von Eggeling Dr. |
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| Affiliation: | 1. Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Medical Faculty, Friedrich Schiller University, Jena, Germany;2. Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research and Infection Biology – Hans‐Kn?ll‐Institute, Jena, Germany;3. Present address: Tecnológico de Monterrey, Departamento de Biotecnologia e Ing. de Alimentos, Ave. E. Garza Sada 2501‐Sur Monterrey, N.L. Mexico, CP 64849.;4. University Hospital, Department of Otorhinolaryngology, Head and Neck Surgery, Medical Faculty, University Cologne, K?ln, Germany;5. Department of Otorhinolaryngology, Medical Faculty, Friedrich Schiller University, Jena, Germany |
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| Abstract: | Human papillomavirus (HPV) infection has been identified as an etiologic agent for a subset of oral squamous cell carcinoma (OSCC) with increasing incidence. HPV DNA‐positivity may confer better prognosis but the related oncogenic mechanisms are unknown. For the identification of HPV relevant proteins, we analyzed microdissected cells from HPV DNA‐positive (n = 17) and HPV DNA‐negative (n = 7) OSCC tissue samples. We identified 18 proteins from tumor tissues by peptide fingerprint mapping and SELDI MS that were separated using 2‐DE. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified thioredoxin (TRX) and epidermal‐fatty acid binding protein as upregulated in HPV related tumor tissue. This study, investigating for the first time proteomic changes in microdissected HPV infected tumor tissue, provides an indication on the oncogenic potential of viruses. |
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| Keywords: | Biomarker Head and neck cancer HPV Immunohistochemistry SELDI‐TOF MS |
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