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LuMPIS – A modified luminescence‐based mammalian interactome mapping pull‐down assay for the investigation of protein–protein interactions encoded by GC‐low ORFs |
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| Authors: | Maria Guadalupe Vizoso Pinto Josefina Maria Villegas Jan Peter Rudolf Haase Jürgen Haas Amelie Sophia Lotz Ania Carolina Muntau Armin Baiker |
| Affiliation: | 1. Max von Pettenkofer‐Institute, Ludwig‐Maximilians University, Munich, Germany;2. Division of Pathway Medicine, University of Edinburgh, Edinburgh, UK;3. Department of Molecular Pediatrics, Children's Research Center, Dr. von Hauner Children's Hospital, Ludwig‐Maximilians University, Munich, Germany |
| Abstract: | The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC‐rich coding sequences of a certain protein are used. In order to study protein–protein interactions in Varicella zoster virus, a GC‐low herpesvirus, we have developed a novel luminescence‐based maltose‐binding protein pull‐down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC‐low ORFs in mammalian expression systems. |
| Keywords: | Peptide sequence tag Protein expression Protein– protein interaction Proteomics methods System biology |